Difference between revisions of "Part:BBa K5143002"
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<h1>Description</h1> | <h1>Description</h1> | ||
<p> | <p> | ||
− | Barnacle, a marine organism, easily stick to underwater matrices through the secretion of various proteins and forming cement complexes. The protein complex for barnacle, Cp19k plays a key role in interfacial adhesion. | + | Barnacle, a marine organism, easily stick to underwater matrices through the secretion of various proteins and forming cement complexes. The protein complex for barnacle, Cp19k plays a key role in interfacial adhesion. Adhesion strenght : 2.2 mJ/m². |
</p> | </p> | ||
<img src="https://static.igem.wiki/teams/5143/bba-k5143002-cp19k.png" width="400" alt="NOM PHOTO"> | <img src="https://static.igem.wiki/teams/5143/bba-k5143002-cp19k.png" width="400" alt="NOM PHOTO"> | ||
<h1>Construction</h1> | <h1>Construction</h1> | ||
<p> | <p> | ||
− | The Cp19k gene was synthesised and its nucleotide sequence optimised for synthesis and expression in Saccharomyces cerevisiae. </p> | + | The Cp19k gene was synthesised and its nucleotide sequence optimised for synthesis and expression in Saccharomyces cerevisiae. This protein is used in fusion with spider silk protein as a bioglue, to improve its adhesive properties : <a href="https://parts.igem.org/Part:BBa_K5143003" target="_blank">BBa_K5143003</a> </p> |
<h1>References</h1> | <h1>References</h1> | ||
<p> | <p> |
Revision as of 15:33, 30 July 2024
Cp19k : a barnacle cement protein used as a bioglue, optimised for Saccharomyces cerevisiae
Description
Barnacle, a marine organism, easily stick to underwater matrices through the secretion of various proteins and forming cement complexes. The protein complex for barnacle, Cp19k plays a key role in interfacial adhesion. Adhesion strenght : 2.2 mJ/m².
Construction
The Cp19k gene was synthesised and its nucleotide sequence optimised for synthesis and expression in Saccharomyces cerevisiae. This protein is used in fusion with spider silk protein as a bioglue, to improve its adhesive properties : BBa_K5143003
References
Malay, A. D., Craig, H. C., Chen, J., Oktaviani, N. A. & Numata, K. Complexity of Spider Dragline Silk. Biomacromolecules 23, 1827–1840 (2022). Multicomponent nature underlies the extraordinary mechanical properties of spider dragline silk | PNAS. https://www-pnas-org.docelec.univ-lyon1.fr/doi/full/10.1073/pnas.2107065118. A bioinspired synthetic fused protein adhesive from barnacle cement and spider dragline for potential biomedical materials - PubMed. https://pubmed.ncbi.nlm.nih.gov/37776922/.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]