Difference between revisions of "Part:BBa K5143002"

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     <h1>Description</h1>
 
     <h1>Description</h1>
 
     <p>
 
     <p>
         Barnacle, a marine organism, easily stick to underwater matrices through the secretion of various proteins and forming cement complexes. The protein complex for barnacle, Cp19k plays a key role in interfacial adhesion.  
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         Barnacle, a marine organism, easily stick to underwater matrices through the secretion of various proteins and forming cement complexes. The protein complex for barnacle, Cp19k plays a key role in interfacial adhesion. Adhesion strenght : 2.2 mJ/m².  
 
     </p>
 
     </p>
 
     <img src="https://static.igem.wiki/teams/5143/bba-k5143002-cp19k.png" width="400" alt="NOM PHOTO">
 
     <img src="https://static.igem.wiki/teams/5143/bba-k5143002-cp19k.png" width="400" alt="NOM PHOTO">
 
     <h1>Construction</h1>
 
     <h1>Construction</h1>
 
     <p>
 
     <p>
The Cp19k gene was synthesised and its nucleotide sequence optimised for synthesis and expression in Saccharomyces cerevisiae.    </p>
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The Cp19k gene was synthesised and its nucleotide sequence optimised for synthesis and expression in Saccharomyces cerevisiae. This protein is used in fusion with spider silk protein as a bioglue, to improve its adhesive properties : <a href="https://parts.igem.org/Part:BBa_K5143003" target="_blank">BBa_K5143003</a>     </p>
 
     <h1>References</h1>
 
     <h1>References</h1>
 
     <p>
 
     <p>

Revision as of 15:33, 30 July 2024


Cp19k : a barnacle cement protein used as a bioglue, optimised for Saccharomyces cerevisiae

Protein Description

Description

Barnacle, a marine organism, easily stick to underwater matrices through the secretion of various proteins and forming cement complexes. The protein complex for barnacle, Cp19k plays a key role in interfacial adhesion. Adhesion strenght : 2.2 mJ/m².

NOM PHOTO

Construction

The Cp19k gene was synthesised and its nucleotide sequence optimised for synthesis and expression in Saccharomyces cerevisiae. This protein is used in fusion with spider silk protein as a bioglue, to improve its adhesive properties : BBa_K5143003

References

Malay, A. D., Craig, H. C., Chen, J., Oktaviani, N. A. & Numata, K. Complexity of Spider Dragline Silk. Biomacromolecules 23, 1827–1840 (2022). Multicomponent nature underlies the extraordinary mechanical properties of spider dragline silk | PNAS. https://www-pnas-org.docelec.univ-lyon1.fr/doi/full/10.1073/pnas.2107065118. A bioinspired synthetic fused protein adhesive from barnacle cement and spider dragline for potential biomedical materials - PubMed. https://pubmed.ncbi.nlm.nih.gov/37776922/.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]