Difference between revisions of "Part:BBa K5226014"

Revision as of 06:23, 29 July 2024

Vib-gcvP

Introduction

One of the goals of iGEM24-SCUT-China-A is to use synthetic biology tools to obtain Halomonas TD strains that can metabolize formate. We chose to introduce the formate assimilation pathway to enable it to utilize formate, a difficult-to-recover product in CDE. As a second approach, based on the homology between Vibrio natriegens and Halomonas TD, we chose to import the C1, C2, and C3 modules from Vibrio natriegens (parts:).

Usage and Biology

Vib-GlyA is serine hydroxymethyltransferase. The protein sequence is fromVibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759. This protein catalyzes the reaction of glycine with 5,10-methylenetetrahydrofolate to form L-serine and tetrahydrofolate during the glycine assimilation(C3M.This part is used in BBa_xx and BBa_xx.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 546
Illegal XbaI site found at 1602
Illegal PstI site found at 2237
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1629
Illegal NheI site found at 2226
Illegal PstI site found at 2237
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 353
Illegal BglII site found at 1638
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 546
Illegal XbaI site found at 1602
Illegal PstI site found at 2237
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 546
Illegal XbaI site found at 1602
Illegal PstI site found at 2237
1000
COMPATIBLE WITH RFC[1000]

@@ Line 7: / Line 7: @@
 ===Usage and Biology===
-Vib-GcvP is glycine dehydrogenase. The protein sequence is fromVibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759. This protein acts in conjunction with GvcH to form H-protein-S-aminomethyldihydrolipoyllysine from glycine during the glycine cleavage system(C2M).This part is used in BBa_xx and BBa_xx.
+Vib-GlyA is serine hydroxymethyltransferase. The protein sequence is fromVibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759. This protein catalyzes the reaction of glycine with 5,10-methylenetetrahydrofolate to form L-serine and tetrahydrofolate during the glycine assimilation(C3M.This part is used in BBa_xx and BBa_xx.
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