Difference between revisions of "Part:BBa K5226014"

 
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<partinfo>BBa_K5226014 short</partinfo>
 
<partinfo>BBa_K5226014 short</partinfo>
  
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===Introduction===
 
One of the goals of iGEM24-SCUT-China-A is to use synthetic biology tools to obtain Halomonas TD strains that can metabolize formate. We chose to introduce the formate assimilation pathway to enable it to utilize formate, a difficult-to-recover product in CDE. As a second approach, based on the homology between Vibrio natriegens and Halomonas TD, we chose to import the C1, C2, and C3 modules from Vibrio natriegens (parts:).
 
One of the goals of iGEM24-SCUT-China-A is to use synthetic biology tools to obtain Halomonas TD strains that can metabolize formate. We chose to introduce the formate assimilation pathway to enable it to utilize formate, a difficult-to-recover product in CDE. As a second approach, based on the homology between Vibrio natriegens and Halomonas TD, we chose to import the C1, C2, and C3 modules from Vibrio natriegens (parts:).
  
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===Usage and Biology===
 
===Usage and Biology===
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Vib-GcvP is glycine dehydrogenase. The protein sequence is fromVibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759. This protein acts in conjunction with GvcH to form H-protein-S-aminomethyldihydrolipoyllysine from glycine during the glycine cleavage system(C2M).This part is used in BBa_xx and BBa_xx.
  
 
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Revision as of 06:15, 29 July 2024


Vib-gcvP

Introduction

One of the goals of iGEM24-SCUT-China-A is to use synthetic biology tools to obtain Halomonas TD strains that can metabolize formate. We chose to introduce the formate assimilation pathway to enable it to utilize formate, a difficult-to-recover product in CDE. As a second approach, based on the homology between Vibrio natriegens and Halomonas TD, we chose to import the C1, C2, and C3 modules from Vibrio natriegens (parts:).

Usage and Biology

Vib-GcvP is glycine dehydrogenase. The protein sequence is fromVibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759. This protein acts in conjunction with GvcH to form H-protein-S-aminomethyldihydrolipoyllysine from glycine during the glycine cleavage system(C2M).This part is used in BBa_xx and BBa_xx.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 546
    Illegal XbaI site found at 1602
    Illegal PstI site found at 2237
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1629
    Illegal NheI site found at 2226
    Illegal PstI site found at 2237
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 353
    Illegal BglII site found at 1638
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 546
    Illegal XbaI site found at 1602
    Illegal PstI site found at 2237
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 546
    Illegal XbaI site found at 1602
    Illegal PstI site found at 2237
  • 1000
    COMPATIBLE WITH RFC[1000]