Difference between revisions of "Part:BBa K5226011"

 
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<partinfo>BBa_K5226011 short</partinfo>
 
<partinfo>BBa_K5226011 short</partinfo>
  
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===Introduction===
 
One of the goals of iGEM24-SCUT-China-A is to use synthetic biology tools to obtain Halomonas TD strains that can metabolize formate. We chose to introduce the formate assimilation pathway to enable it to utilize formate, a difficult-to-recover product in CDE. As a second approach, based on the homology between Vibrio natriegens and Halomonas TD, we chose to import the C1, C2, and C3 modules from Vibrio natriegens (parts:).
 
One of the goals of iGEM24-SCUT-China-A is to use synthetic biology tools to obtain Halomonas TD strains that can metabolize formate. We chose to introduce the formate assimilation pathway to enable it to utilize formate, a difficult-to-recover product in CDE. As a second approach, based on the homology between Vibrio natriegens and Halomonas TD, we chose to import the C1, C2, and C3 modules from Vibrio natriegens (parts:).
  
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===Usage and Biology===
 
===Usage and Biology===
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Vib-Lpd is dihydrolipoamide dehydrogenase. The protein sequence is fromVibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759. This protein catalyzes the conversion of dihydrolipoyllysine into lipoyllysine during the glycine cleavage system(C2M). This part is used in BBa_xx and BBa_xx.
  
 
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Revision as of 05:52, 29 July 2024


Vib-lpd

Introduction

One of the goals of iGEM24-SCUT-China-A is to use synthetic biology tools to obtain Halomonas TD strains that can metabolize formate. We chose to introduce the formate assimilation pathway to enable it to utilize formate, a difficult-to-recover product in CDE. As a second approach, based on the homology between Vibrio natriegens and Halomonas TD, we chose to import the C1, C2, and C3 modules from Vibrio natriegens (parts:).

Usage and Biology

Vib-Lpd is dihydrolipoamide dehydrogenase. The protein sequence is fromVibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759. This protein catalyzes the conversion of dihydrolipoyllysine into lipoyllysine during the glycine cleavage system(C2M). This part is used in BBa_xx and BBa_xx.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 397
    Illegal SpeI site found at 478
    Illegal PstI site found at 191
    Illegal PstI site found at 668
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 397
    Illegal NheI site found at 942
    Illegal NheI site found at 1060
    Illegal SpeI site found at 478
    Illegal PstI site found at 191
    Illegal PstI site found at 668
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 397
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 397
    Illegal SpeI site found at 478
    Illegal PstI site found at 191
    Illegal PstI site found at 668
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 397
    Illegal SpeI site found at 478
    Illegal PstI site found at 191
    Illegal PstI site found at 668
  • 1000
    COMPATIBLE WITH RFC[1000]