Difference between revisions of "Part:BBa K5226010"
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<partinfo>BBa_K5226010 short</partinfo> | <partinfo>BBa_K5226010 short</partinfo> | ||
| + | ===Introduction=== | ||
One of the goals of iGEM24-SCUT-China-A is to use synthetic biology tools to obtain Halomonas TD strains that can metabolize formate. We chose to introduce the formate assimilation pathway to enable it to utilize formate, a difficult-to-recover product in CDE. As a second approach, based on the homology between Vibrio natriegens and Halomonas TD, we chose to import the C1, C2, and C3 modules from Vibrio natriegens (parts:). | One of the goals of iGEM24-SCUT-China-A is to use synthetic biology tools to obtain Halomonas TD strains that can metabolize formate. We chose to introduce the formate assimilation pathway to enable it to utilize formate, a difficult-to-recover product in CDE. As a second approach, based on the homology between Vibrio natriegens and Halomonas TD, we chose to import the C1, C2, and C3 modules from Vibrio natriegens (parts:). | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | Vib-FolD is methenyltetrahydrofolate cyclohydrolase. The protein sequence is fromVibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759. This protein is bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase, catalyzing the conversion of 10-formyltetrahydrofolate into 5,10-methenyltetrahydrofolate and then convert to 5,10-methylene-5,6,7,8-tetrahydrofolate during the tetrahydrofolate (THF) cycle(C1M). This part is used in BBa_xx and BBa_xx. | ||
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Revision as of 03:20, 29 July 2024
Vib-folD
Introduction
One of the goals of iGEM24-SCUT-China-A is to use synthetic biology tools to obtain Halomonas TD strains that can metabolize formate. We chose to introduce the formate assimilation pathway to enable it to utilize formate, a difficult-to-recover product in CDE. As a second approach, based on the homology between Vibrio natriegens and Halomonas TD, we chose to import the C1, C2, and C3 modules from Vibrio natriegens (parts:).
Usage and Biology
Vib-FolD is methenyltetrahydrofolate cyclohydrolase. The protein sequence is fromVibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759. This protein is bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase, catalyzing the conversion of 10-formyltetrahydrofolate into 5,10-methenyltetrahydrofolate and then convert to 5,10-methylene-5,6,7,8-tetrahydrofolate during the tetrahydrofolate (THF) cycle(C1M). This part is used in BBa_xx and BBa_xx.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 237
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]