Difference between revisions of "Part:BBa K5226006"

 
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<partinfo>BBa_K5226006 short</partinfo>
 
<partinfo>BBa_K5226006 short</partinfo>
  
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===Introduction===
 
One of the goals of iGEM24-SCUT-China-A is to use synthetic biology tools to obtain Halomonas TD strains that can metabolize formate. We chose to introduce the formate assimilation pathway to enable it to utilize formate, a difficult-to-recover product in CDE. For the first method, based on previous studies obtained from literature research, we selected the tetrahydrofolate (THF) cycle imported from Methylobacterium extorquens AM1(parts:) and strengthened the endogenous C2 and C3 modules of Halomonas TD(parts:).
 
One of the goals of iGEM24-SCUT-China-A is to use synthetic biology tools to obtain Halomonas TD strains that can metabolize formate. We chose to introduce the formate assimilation pathway to enable it to utilize formate, a difficult-to-recover product in CDE. For the first method, based on previous studies obtained from literature research, we selected the tetrahydrofolate (THF) cycle imported from Methylobacterium extorquens AM1(parts:) and strengthened the endogenous C2 and C3 modules of Halomonas TD(parts:).
  
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===Usage and Biology===
 
===Usage and Biology===
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TD-GcvP is glycine dehydrogenase.The protein sequence is from Halomonas sp. TD01 strain.The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO2 is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein during the glycine cleavage system(C2M) . This part is used in BBa_xx and BBa_xx.
  
 
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Revision as of 01:49, 28 July 2024


TD-gcvP

Introduction

One of the goals of iGEM24-SCUT-China-A is to use synthetic biology tools to obtain Halomonas TD strains that can metabolize formate. We chose to introduce the formate assimilation pathway to enable it to utilize formate, a difficult-to-recover product in CDE. For the first method, based on previous studies obtained from literature research, we selected the tetrahydrofolate (THF) cycle imported from Methylobacterium extorquens AM1(parts:) and strengthened the endogenous C2 and C3 modules of Halomonas TD(parts:).

Usage and Biology

TD-GcvP is glycine dehydrogenase.The protein sequence is from Halomonas sp. TD01 strain.The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO2 is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein during the glycine cleavage system(C2M) . This part is used in BBa_xx and BBa_xx.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1564
    Illegal PstI site found at 1841
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1662
    Illegal SpeI site found at 1564
    Illegal PstI site found at 1841
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1564
    Illegal PstI site found at 1841
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1564
    Illegal PstI site found at 1841
    Illegal NgoMIV site found at 291
    Illegal NgoMIV site found at 1636
  • 1000
    COMPATIBLE WITH RFC[1000]