Difference between revisions of "Part:BBa K5226000"

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<partinfo>BBa_K5226000 short</partinfo>
 
<partinfo>BBa_K5226000 short</partinfo>
  
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===Introduction===
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One of the goals of iGEM24-SCUT-China-A is to use synthetic biology tools to obtain Halomonas TD strains that can metabolize formate. We chose to introduce the formate assimilation pathway to enable it to utilize formate, a difficult-to-recover product in CDE. For the first method, based on previous studies obtained from literature research, we selected the tetrahydrofolate (THF) cycle imported from Methylobacterium extorquens AM1(parts:) and strengthened the endogenous C2 and C3 modules of Halomonas TD(parts:).
  
 
===Usage and Biology===
 
===Usage and Biology===
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AM1-FHS is Formate-tetrahydrofolate ligase. The protein sequence is from Methylobacterium extorquens AM1. This protein catalyzes the conversion of formate into 10-formyltetrahydrofolate during the tetrahydrofolate (THF) cycle . This part is used in BBa_xx and BBa_xx
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AM1-Ftfl is Formate-tetrahydrofolate ligase. The protein sequence is from Methylobacterium extorquens AM1. This protein catalyzes the conversion of formate into 10-formyltetrahydrofolate during the tetrahydrofolate (THF) cycle(C1M) . This part is used in BBa_xx and BBa_xx
  
 
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Revision as of 11:27, 27 July 2024


AM1-ftfl

Introduction

One of the goals of iGEM24-SCUT-China-A is to use synthetic biology tools to obtain Halomonas TD strains that can metabolize formate. We chose to introduce the formate assimilation pathway to enable it to utilize formate, a difficult-to-recover product in CDE. For the first method, based on previous studies obtained from literature research, we selected the tetrahydrofolate (THF) cycle imported from Methylobacterium extorquens AM1(parts:) and strengthened the endogenous C2 and C3 modules of Halomonas TD(parts:).

Usage and Biology

AM1-Ftfl is Formate-tetrahydrofolate ligase. The protein sequence is from Methylobacterium extorquens AM1. This protein catalyzes the conversion of formate into 10-formyltetrahydrofolate during the tetrahydrofolate (THF) cycle(C1M) . This part is used in BBa_xx and BBa_xx

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 379
    Illegal AgeI site found at 693
    Illegal AgeI site found at 721
    Illegal AgeI site found at 859
  • 1000
    COMPATIBLE WITH RFC[1000]