Difference between revisions of "Part:BBa K5133000"

Revision as of 02:46, 27 July 2024

T7 promoter (from plasmid pJL1)

Group: GEC-China (iGEM 2024, team number: #5133)

This basic part is derived from plasmid pJL1 (Addgene: #69496)^[1], including a conserved DNA sequence of T7 promoter as 5'-taatacgactcactatagggaga-3'^[2]. The plasmid pJL1 is commonly used for the sfGFP expression for cell-free protein synthesis (CFPS). Hence, this part is used for the construction of three composite parts: BBa_K5133004 (sfGFP generator), BBa_K5133006 (Microcin H47 generator), and BBa_K5133008 (Microcin M generator), for CFPS in our project.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 51
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 51
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 51
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 32

@@ Line 5: / Line 5: @@
 Group: <b>GEC-China (iGEM 2024, team number: #5133)</b>
-This basic part is derived from plasmid pJL1 (Addgene: #69496)<sup>[1]</sup>, including a conserved DNA sequence of T7 promoter as <b>5'-taatacgactcactatagggaga-3'</b><sup>[2]</sup>. The plasmid pJL1 is commonly used for the sfGFP expression for cell-free protein synthesis (CFPS). Hence, this part is used for the construction of three composite parts: BBa_K5133004 (sfGFP generator for CFPS), BBa_K5133006 (Microcin H47 generator for CFPS), and BBa_K5133008 (Microcin M generator for CFPS), respectively.
+This basic part is derived from plasmid pJL1 (Addgene: #69496)<sup>[1]</sup>, including a conserved DNA sequence of T7 promoter as <b>5'-taatacgactcactatagggaga-3'</b><sup>[2]</sup>. The plasmid pJL1 is commonly used for the sfGFP expression for cell-free protein synthesis (CFPS). Hence, this part is used for the construction of three composite parts: <bbpart>BBa_K5133004</bbpart> (sfGFP generator), <bbpart>BBa_K5133006</bbpart> (Microcin H47 generator), and <bbpart>BBa_K5133008</bbpart> (Microcin M generator), for CFPS in our project.