Difference between revisions of "Part:BBa K4615006:Design"
| Line 9: | Line 9: | ||
We obtained this part by introducing 20 bp of sgRNA within the tpiA genome that guides the Cas9 protein to cut and create strand breaks. | We obtained this part by introducing 20 bp of sgRNA within the tpiA genome that guides the Cas9 protein to cut and create strand breaks. | ||
| + | Annealing Temperature: 48C | ||
| + | GC:35% | ||
===Source=== | ===Source=== | ||
Latest revision as of 15:59, 12 October 2023
Reverse primer to insert 20 bp of sgRNA of frdA into pTargetF
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We obtained this part by introducing 20 bp of sgRNA within the tpiA genome that guides the Cas9 protein to cut and create strand breaks.
Annealing Temperature: 48C
GC:35%
Source
The 20bp of sgRNA was generated using CHOPCHOP and analyzed based on various factors such as GC content, self-complementarity and efficiency.