Difference between revisions of "Part:BBa K4725001"
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PsinI is an endogenous promoter in B. subtilis. It is activated by phosphorylated spo0A, a master regulator in B. subtilis. Its expression can be induced by potassium efflux using potassium ionophores such as valinomycin and cereulide. In our case, 0.3 µM of valinomycin is enough to trigger this promoter when B. subtilis is grown in BHI medium with pH 8.5. | PsinI is an endogenous promoter in B. subtilis. It is activated by phosphorylated spo0A, a master regulator in B. subtilis. Its expression can be induced by potassium efflux using potassium ionophores such as valinomycin and cereulide. In our case, 0.3 µM of valinomycin is enough to trigger this promoter when B. subtilis is grown in BHI medium with pH 8.5. |
Revision as of 15:58, 12 October 2023
No part name specified with partinfo tag.
PsinI is an endogenous promoter in B. subtilis. It is activated by phosphorylated spo0A, a master regulator in B. subtilis. Its expression can be induced by potassium efflux using potassium ionophores such as valinomycin and cereulide. In our case, 0.3 µM of valinomycin is enough to trigger this promoter when B. subtilis is grown in BHI medium with pH 8.5.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]