Difference between revisions of "Part:BBa K4653121"

Latest revision as of 15:57, 12 October 2023

SacB promoter - SpoVG RBS - MazE/F - Fd Terminator

SacB promoter is a sucrose induced promoter from Bacillus subtilis. MazEF is one of the most common Toxin-antitoxin systems (TA system), belonging to the kid family of the Class II TA classification system, and was first identified in E. coli. While mazF is a stable toxic protein capable of non-specifically cutting intracellular RNA and causing cell death, MazE is an unstable antitoxin that antagonizes MazF and can be degraded by the ATP-dependent ClpPa serine protease in cells. mazE is less stable than mazF, so cells must be able to survive when mazE is consistently expressed. And we needed a terminator to help us stop the above transcription, so we chose the fd terminator, which is the central terminator of bacteriophage fd. Additionally, we repeated the fd terminator as a strong terminator.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 962
Illegal AgeI site found at 682
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1231
Illegal SapI.rc site found at 1175

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4653121 short</partinfo>
-SacB promoter is a sucrose induced promoter from Bacillus subtilis. MazEF is one of the most common Toxin-antitoxin systems (TA system), belonging to the kid family of the Class II TA classification system, and was first identified in E. coli.
+SacB promoter is a sucrose induced promoter from Bacillus subtilis. MazEF is one of the most common Toxin-antitoxin systems (TA system), belonging to the kid family of the Class II TA classification system, and was first identified in <i>E. coli</i>.
 While mazF is a stable toxic protein capable of non-specifically cutting intracellular RNA and causing cell death, MazE is an unstable antitoxin that antagonizes MazF and can be degraded by the ATP-dependent ClpPa serine protease in cells. mazE is less stable than mazF, so cells must be able to survive when mazE is consistently expressed. And we needed a terminator to help us stop the above transcription, so we chose the fd terminator, which is the central terminator of bacteriophage fd. Additionally, we repeated the fd terminator as a strong terminator.