Difference between revisions of "Part:BBa K4725001"
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===Usage and Biology===
 
  
 
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Revision as of 15:57, 12 October 2023


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PsinI is an endogenous promoter in B. subtilis. It is activated by phosphorylated spo0A, a master regulator in B. subtilis. Its expression can be induced by potassium efflux using potassium ionophores such as valinomycin and cereulide. In our case, 0.3 µM of valinomycin is enough to trigger this promoter when B. subtilis is grown in BHI medium with pH 8.5.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]