Difference between revisions of "Part:BBa K176000"

(→‎MEASUREMENT)
Line 14: Line 14:
 
K176000, the hybrid promoter，is the heart of our project. To investigate the response of K176000, we performed a lot of measurement by building different devices. At last, we obtain a lot of data which can show the response of K176000 to time, AHL, aTc and tetR.
 
K176000, the hybrid promoter，is the heart of our project. To investigate the response of K176000, we performed a lot of measurement by building different devices. At last, we obtain a lot of data which can show the response of K176000 to time, AHL, aTc and tetR.
  
−
===AHL-->K176000===
+
===AHL&time-->K176000===
 +
 
 
K176026[https://parts.igem.org/wiki/index.php?title=Part:BBa_K176026]: pCon 0.70->luxR+pLux/Tet->GFP
 
K176026[https://parts.igem.org/wiki/index.php?title=Part:BBa_K176026]: pCon 0.70->luxR+pLux/Tet->GFP
  
Line 22: Line 23:
  
 
K176130[https://parts.igem.org/wiki/index.php?title=Part:BBa_K176130]: pCon 0.04->luxR+pLux/Tet->GFP
 
K176130[https://parts.igem.org/wiki/index.php?title=Part:BBa_K176130]: pCon 0.04->luxR+pLux/Tet->GFP
 +
 +
===AHL&aTc-->K176000===
 +
 +
K176071[https://parts.igem.org/wiki/index.php?title=Part:BBa_K176071#MEASUREMENT]: pCon 0.36->tetR-LVA+pCon 0.70->luxR+pLux/Tet->GFP
 +
 +
K176075[https://parts.igem.org/wiki/index.php?title=Part:BBa_K176075#MEASUREMENT]: pCon 0.15->tetR-LVA+pCon 0.70->luxR+pLux/Tet->GFP
 +
 +
K176078[https://parts.igem.org/wiki/index.php?title=Part:BBa_K176078#MEASUREMENT]: pCon 0.01->tetR+pCon 0.70->luxR+pLux/Tet->GFP
 +
  
  

Revision as of 02:36, 21 October 2009

pLux/Tet Hybrid Promoter: (LuxR+,TetR-)->PoPS

sense two inputs, activated by LuxR/3OC6HSL and repressed by tetR

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

MEASUREMENT

K176000, the hybrid promoter，is the heart of our project. To investigate the response of K176000, we performed a lot of measurement by building different devices. At last, we obtain a lot of data which can show the response of K176000 to time, AHL, aTc and tetR.

AHL&time-->K176000

K176026[1]: pCon 0.70->luxR+pLux/Tet->GFP

K176126[2]: pCon 0.36->luxR+pLux/Tet->GFP

K176128[3]: pCon 0.15->luxR+pLux/Tet->GFP

K176130[4]: pCon 0.04->luxR+pLux/Tet->GFP

AHL&aTc-->K176000

K176071[5]: pCon 0.36->tetR-LVA+pCon 0.70->luxR+pLux/Tet->GFP

K176075[6]: pCon 0.15->tetR-LVA+pCon 0.70->luxR+pLux/Tet->GFP

K176078[7]: pCon 0.01->tetR+pCon 0.70->luxR+pLux/Tet->GFP