Difference between revisions of "Part:BBa K4757999:Design"
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===Source=== | ===Source=== | ||
− | XylS/Pm was stems from the TOL plasmid in Pseudomonas putida (obtained through the pSEVA438) | + | <html> |
− | The sRNA scaffold (SgrS) is natively found in Escherichia coli as part from a sugar transport regulatory network | + | <p>XylS/Pm was stems from the TOL plasmid in Pseudomonas putida (obtained through the pSEVA438);<br> |
− | AlkS/pAlkB is natively found in Pseudomonas putida (sequence ordered through gene synthesis). | + | The sRNA scaffold (SgrS) is natively found in Escherichia coli as part from a sugar transport regulatory network;<br> |
− | mKate2 originiated from Entacmaea quadricolor (sequence ordered through gene synthesis). | + | AlkS/pAlkB is natively found in Pseudomonas putida (sequence ordered through gene synthesis).<br> |
+ | mKate2 originiated from Entacmaea quadricolor (sequence ordered through gene synthesis).</p> | ||
+ | </html> | ||
===References=== | ===References=== |
Revision as of 15:45, 12 October 2023
Synthetic expression cassette regulated by terepthalic acid and alkanes for PET and PE sensing
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Illegal EcoRI site found at 4298 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 4202
Illegal EcoRI site found at 4298
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Design Notes
Source
XylS/Pm was stems from the TOL plasmid in Pseudomonas putida (obtained through the pSEVA438);
The sRNA scaffold (SgrS) is natively found in Escherichia coli as part from a sugar transport regulatory network;
AlkS/pAlkB is natively found in Pseudomonas putida (sequence ordered through gene synthesis).
mKate2 originiated from Entacmaea quadricolor (sequence ordered through gene synthesis).
References
Bao, T., Qian, Y., Xin, Y., Collins, J. J., & Lu, T. (2023). Engineering microbial division of labor for plastic upcycling. Nature communications, 14(1), 5712. <a href="https://doi.org/10.1038/s41467-023-40777-x">https://doi.org/10.1038/s41467-023-40777-x</a>
Chen, D., Xu, S., Li, S., Tao, S., Li, L., Chen, S., & Wu, L. (2023). Directly Evolved AlkS-Based Biosensor Platform for Monitoring and High-Throughput Screening of Alkane Production. ACS synthetic biology, 12(3), 832-841. <a href="https://doi.org/10.1021/acssynbio.2c00620">https://doi.org/10.1021/acssynbio.2c00620</a>
Gallegos, M. T., Marqués, S., & Ramos, J. L. (1996). Expression of the tol plasmid xylS gene in pseudomonas putida occurs from a alpha 70-dependent promoter or from alpha 70- and Alpha 54-dependent tandem promoters according to the compound used for Growth. Journal of Bacteriology, 178(8), 2356-2361. https://doi.org/10.1128/jb.178.8.2356-2361.1996
Gawin, A., Valla, S., & Brautaset, T. (2017). The XylS/Pm regulator/promoter system and its use in fundamental studies of bacterial gene expression, recombinant protein production and metabolic engineering. Microbial biotechnology, 10(4), 702-718. <a href="https://doi.org/10.1111/1751-7915.12701">https://doi.org/10.1111/1751-7915.12701</a>
<a>Geyer, R., Jambeck, J. R., & Law, K. L. (2017). Production, use, and fate of all plastics ever made.</a> Science advances, 3(7), e1700782. <a href="https://doi.org/10.1126/sciadv.1700782">https://doi.org/10.1126/sciadv.1700782</a>
Gottesman S. (2004). The small RNA regulators of Escherichia coli: roles and mechanisms*. Annual review of microbiology, 58, 303-328.</a> <a href="https://doi.org/10.1146/annurev.micro.58.030603.123841">https://doi.org/10.1146/annurev.micro.58.030603.123841</a>
Kelly, C. L., Harris, A. W. K., Steel, H., Hancock, E. J., Heap, J. T., & Papachristodoulou, A. (2018). Synthetic negative feedback circuits using engineered small RNAs. Nucleic acids research, 46(18), 9875-9889. <a href="https://doi.org/10.1093/nar/gky828">https://doi.org/10.1093/nar/gky828</a>
Li, J., Nina, M. R. H., Zhang, X., & Bai, Y. (2022). Engineering Transcription Factor XylS for Sensing Phthalic Acid and Terephthalic Acid: An Application for Enzyme Evolution. ACS synthetic biology, 11(3), 1106-1113. <a href="https://doi.org/10.1021/acssynbio.1c00275">https://doi.org/10.1021/acssynbio.1c00275</a>
Lu, H., Diaz, D. J., Czarnecki, N. J., Zhu, C., Kim, W., Shroff, R., Acosta, D. J., Alexander, B. R., Cole, H. O., Zhang, Y., Lynd, N. A., Ellington, A. D., & Alper, H. S. (2022). Machine learning-aided engineering of hydrolases for PET depolymerization. Nature, 604(7907), 662-667. <a href="https://doi.org/10.1038/s41586-022-04599-z">https://doi.org/10.1038/s41586-022-04599-z</a>
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Møller, T., Franch, T., Højrup, P., Keene, D. R., Bächinger, H. P., Brennan, R. G., & Valentin-Hansen, P. (2002). Hfq: a bacterial Sm-like protein that mediates RNA-RNA interaction. Molecular cell, 9(1), 23-30. <a href="https://doi.org/10.1016/s1097-2765(01)00436-1">https://doi.org/10.1016/s1097-2765(01)00436-1</a>
Na, D., Yoo, S. M., Chung, H., Park, H., Park, J. H., & Lee, S. Y. (2013). Metabolic engineering of Escherichia coli using synthetic small regulatory RNAs. Nature biotechnology, 31(2), 170-174. <a href="https://doi.org/10.1038/nbt.2461">https://doi.org/10.1038/nbt.2461</a>
Sharma, S.R. (2018). Bioremediation of Polythenes and Plastics: A Microbial Approach. In: Prasad, R., Aranda, E. (eds) Approaches in Bioremediation. Nanotechnology in the Life Sciences. Springer, Cham. <a href="https://doi.org/10.1007/978-3-030-02369-0_6">https://doi.org/10.1007/978-3-030-02369-0_6</a>
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