Difference between revisions of "Part:BBa K176000"
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K176000 SequenceAndFeatures</partinfo> | <partinfo>BBa_K176000 SequenceAndFeatures</partinfo> | ||
+ | |||
+ | ==MEASUREMENT== | ||
+ | K176000, the hybrid promoter,is the heart of our project. To investigate the response of K176000, we performed a lot of measurement by building different devices. At last, we obtain a lot of data which can show the response of K176000 to time, AHL, aTc and tetR. | ||
+ | |||
+ | ===AHL-->K176000=== | ||
+ | K176026[https://parts.igem.org/wiki/index.php?title=Part:BBa_K176026]: pCon 0.70->luxR+pLux/Tet->GFP | ||
+ | |||
+ | K176126[https://parts.igem.org/wiki/index.php?title=Part:BBa_K176126#MEASUREMENT1]: pCon 0.36->luxR+pLux/Tet->GFP | ||
+ | |||
+ | K176128[https://parts.igem.org/wiki/index.php?title=Part:BBa_K176128]: pCon 0.15->luxR+pLux/Tet->GFP | ||
+ | |||
+ | K176130[https://parts.igem.org/wiki/index.php?title=Part:BBa_K176130]: pCon 0.04->luxR+pLux/Tet->GFP | ||
Revision as of 02:21, 21 October 2009
pLux/Tet Hybrid Promoter: (LuxR+,TetR-)->PoPS
sense two inputs, activated by LuxR/3OC6HSL and repressed by tetR
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
MEASUREMENT
K176000, the hybrid promoter,is the heart of our project. To investigate the response of K176000, we performed a lot of measurement by building different devices. At last, we obtain a lot of data which can show the response of K176000 to time, AHL, aTc and tetR.
AHL-->K176000
K176026[1]: pCon 0.70->luxR+pLux/Tet->GFP
K176126[2]: pCon 0.36->luxR+pLux/Tet->GFP
K176128[3]: pCon 0.15->luxR+pLux/Tet->GFP
K176130[4]: pCon 0.04->luxR+pLux/Tet->GFP