Difference between revisions of "Part:BBa K4765113"
| Line 7: | Line 7: | ||
===Introduction=== | ===Introduction=== | ||
| − | This composite part is composed of ''Hypsibius exemplaris'' mitochondrial single-stranded DNA binding protein (H. ex mtSSB) and improved ribozyme-assisted polycistronic co-expression system：pRAP. H. ex mtSSB can coat ssDNA to limit damage during desiccation and UV radiation. | + | This composite part is composed of ''Hypsibius exemplaris'' mitochondrial single-stranded DNA binding protein (''H. ex'' mtSSB) and improved ribozyme-assisted polycistronic co-expression system：pRAP. ''H. ex'' mtSSB can coat ssDNA to limit damage during desiccation and UV radiation. |
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 15:37, 12 October 2023
Twister P1 + T7_RBS + H. ex mtSSB + stem-loop
Introduction
This composite part is composed of Hypsibius exemplaris mitochondrial single-stranded DNA binding protein (H. ex mtSSB) and improved ribozyme-assisted polycistronic co-expression system：pRAP. H. ex mtSSB can coat ssDNA to limit damage during desiccation and UV radiation.
Usage and Biology
We constructed SAHS 33020 into pRAP to endow E. coli with desiccation-resistant and anti-UV capability. And this composite part can cooperate with BBa_K4765112(ribozyme+T7_RBS+SAHS 33020+stem-loop) to increase the survival rate of E.coli under desiccation.
Characterization
Get details in BBa_K4765016.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 247
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 515
Illegal BsaI.rc site found at 596
Illegal SapI site found at 409