Difference between revisions of "Part:BBa K4614107"
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<partinfo>BBa_K4614107 short</partinfo> | <partinfo>BBa_K4614107 short</partinfo> | ||
| − | Using the strong promoter T7, we express the quorum sensing regulatory protein LuxR and regulate the expression of the fluorescent protein gene superfold GFP. When this pathway is introduced into the host bacterium E. coli and operates normally, upon reaching a threshold concentration of exogenously added high acyl-homoserine lactone (AHL or HSL) signal molecules, it forms a complex with LuxR. This complex activates the promoter transcription encoding luciferase, and green fluorescence can be observed. | + | Using the strong promoter T7, we express the quorum sensing regulatory protein LuxR and regulate the expression of the fluorescent protein gene superfold GFP. When this pathway is introduced into the host bacterium <i>E. coli</i> and operates normally, upon reaching a threshold concentration of exogenously added high acyl-homoserine lactone (AHL or HSL) signal molecules, it forms a complex with LuxR. This complex activates the promoter transcription encoding luciferase, and green fluorescence can be observed. |
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| − | <span class='h3bb'>Sequence and Features</span> | + | ===<span class='h3bb'>Sequence and Features</span>=== |
<partinfo>BBa_K4614107 SequenceAndFeatures</partinfo> | <partinfo>BBa_K4614107 SequenceAndFeatures</partinfo> | ||
Latest revision as of 15:36, 12 October 2023
T7 Promoter-RBS-LuxR-B0015 Ter+Plux-RBS-sfGFP-B0015 Ter
Using the strong promoter T7, we express the quorum sensing regulatory protein LuxR and regulate the expression of the fluorescent protein gene superfold GFP. When this pathway is introduced into the host bacterium E. coli and operates normally, upon reaching a threshold concentration of exogenously added high acyl-homoserine lactone (AHL or HSL) signal molecules, it forms a complex with LuxR. This complex activates the promoter transcription encoding luciferase, and green fluorescence can be observed.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 969