Difference between revisions of "Part:BBa K4948033"
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One of the plaamids comprising our biosensor, MoNitro, continuously produces GFP under normal conditions under the control of the PJEx-D promoter. However, when exposed to high concentrations of nitrate, the expression of the Mf_lon protease and EilR repressor, which are regulated by the nitrate inducible promoter, is overwhelmed, resulting in the degradation of lva-tagged GFP, and the transcription of the PJEx-D promoter is inhibited by EilR, resulting in low GFP levels.
 
One of the plaamids comprising our biosensor, MoNitro, continuously produces GFP under normal conditions under the control of the PJEx-D promoter. However, when exposed to high concentrations of nitrate, the expression of the Mf_lon protease and EilR repressor, which are regulated by the nitrate inducible promoter, is overwhelmed, resulting in the degradation of lva-tagged GFP, and the transcription of the PJEx-D promoter is inhibited by EilR, resulting in low GFP levels.
  
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===Usage and Biology===
 
===Usage and Biology===
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==Vector Map==
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https://static.igem.wiki/teams/4948/wiki/parts/contribution/pnitrate-safe-green-map.jpg
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Revision as of 15:29, 12 October 2023


PJEx-D-RBS-vsfGFP-0_lva

One of the plaamids comprising our biosensor, MoNitro, continuously produces GFP under normal conditions under the control of the PJEx-D promoter. However, when exposed to high concentrations of nitrate, the expression of the Mf_lon protease and EilR repressor, which are regulated by the nitrate inducible promoter, is overwhelmed, resulting in the degradation of lva-tagged GFP, and the transcription of the PJEx-D promoter is inhibited by EilR, resulting in low GFP levels.

Usage and Biology

Vector Map

pnitrate-safe-green-map.jpg


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 199
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 199
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 199
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 199
    Illegal NgoMIV site found at 451
    Illegal AgeI site found at 557
    Illegal AgeI site found at 971
  • 1000
    COMPATIBLE WITH RFC[1000]