Difference between revisions of "Part:BBa K4948033"
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One of the plaamids comprising our biosensor, MoNitro, continuously produces GFP under normal conditions under the control of the PJEx-D promoter. However, when exposed to high concentrations of nitrate, the expression of the Mf_lon protease and EilR repressor, which are regulated by the nitrate inducible promoter, is overwhelmed, resulting in the degradation of lva-tagged GFP, and the transcription of the PJEx-D promoter is inhibited by EilR, resulting in low GFP levels. | One of the plaamids comprising our biosensor, MoNitro, continuously produces GFP under normal conditions under the control of the PJEx-D promoter. However, when exposed to high concentrations of nitrate, the expression of the Mf_lon protease and EilR repressor, which are regulated by the nitrate inducible promoter, is overwhelmed, resulting in the degradation of lva-tagged GFP, and the transcription of the PJEx-D promoter is inhibited by EilR, resulting in low GFP levels. | ||
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Revision as of 15:28, 12 October 2023
PJEx-D-RBS-vsfGFP-0_lva
One of the plaamids comprising our biosensor, MoNitro, continuously produces GFP under normal conditions under the control of the PJEx-D promoter. However, when exposed to high concentrations of nitrate, the expression of the Mf_lon protease and EilR repressor, which are regulated by the nitrate inducible promoter, is overwhelmed, resulting in the degradation of lva-tagged GFP, and the transcription of the PJEx-D promoter is inhibited by EilR, resulting in low GFP levels.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 199
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 199
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 199
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 199
Illegal NgoMIV site found at 451
Illegal AgeI site found at 557
Illegal AgeI site found at 971 - 1000COMPATIBLE WITH RFC[1000]