Difference between revisions of "Part:BBa K177038"

Revision as of 01:07, 21 October 2009

Bistable switch 1 testing device

Bistable switch based on termosensitive lambda repressor (cIts) and lactose operon inhibitor (lacI).

Usage and Biology

The state of the switch is set either by IPTG or temperature. In 0,1 uM IPTG lacI gets inactivated ant transcription from Plac starts. It results in production of cIts and other genes belonging to this part of switch (in this case GFP). After removal of IPTG the state remains stable - P_Rm is repressed by cIts and genes under Plac are expressed. In 42^oC cIts gets inactivated and genes under P_Rm (in this case lacI and RFP) are expressed. Because lacI is expressed to Plac gets repressed. The state remains stable after transfer of this device to 30^oC. State of the device can be switched by pulses of IPTG and 42^oC. Temperature of stable work is 30^oC.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 3058
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 3645
Illegal AgeI site found at 3757
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1614

@@ Line 8: / Line 8: @@
 ===Usage and Biology===
-The state of the switch is set either by IPTG or temperature. In 0,1 uM IPTG lacI gets inactivated ant transcription from Plac starts. It results in production of cIts and other genes belonging to this part of switch (in this case GFP). After removal of IPTG the state remains stable - P<sub>Rm</sub> is repressed by cIts and genes under Plac are expressed. In 42<sup>o</sup>C cIts gets inactivated and expression of genes under P<sub>Rm</sub> (in this case lacI and RFP) are expressed. Because lacI is expressed to Plac gets repressed. The state remains stable after transfer of this device to 30<sup>o</sup>C. State of the device can be switched by pulses of IPTG and 42<sup>o</sup>C. Temperature of stable work is 30<sup>o</sup>C.
+The state of the switch is set either by IPTG or temperature. In 0,1 uM IPTG lacI gets inactivated ant transcription from Plac starts. It results in production of cIts and other genes belonging to this part of switch (in this case GFP). After removal of IPTG the state remains stable - P<sub>Rm</sub> is repressed by cIts and genes under Plac are expressed. In 42<sup>o</sup>C cIts gets inactivated and genes under P<sub>Rm</sub> (in this case lacI and RFP) are expressed. Because lacI is expressed to Plac gets repressed. The state remains stable after transfer of this device to 30<sup>o</sup>C. State of the device can be switched by pulses of IPTG and 42<sup>o</sup>C. Temperature of stable work is 30<sup>o</sup>C.
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