Difference between revisions of "Part:BBa K227008:Design"

 
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<partinfo>BBa_K227008 short</partinfo>
 
<partinfo>BBa_K227008 short</partinfo>
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===Design Notes===
 
===Design Notes===
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no evidence of ompC region having a natural binding promotor in R. sphaeroides
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There is no evidence of ompC region having a natural binding promotor in R. sphaeroides, so this part requires an OmpR coding region elsewhere to function.
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As a synthesized part, this is not a true composite of BBa_R0082 (ompC) and BBa_K227006 (pucBA), but also includes ~40 bases upstream of the pucBA transcription start site, which includes the putative RBS region of R. sphaeroides.
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===Source===
 
===Source===

Latest revision as of 00:00, 21 October 2009

ompC Promoter+pucBA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There is no evidence of ompC region having a natural binding promotor in R. sphaeroides, so this part requires an OmpR coding region elsewhere to function. As a synthesized part, this is not a true composite of BBa_R0082 (ompC) and BBa_K227006 (pucBA), but also includes ~40 bases upstream of the pucBA transcription start site, which includes the putative RBS region of R. sphaeroides.

Source

existing registry parts

References