Difference between revisions of "Part:BBa K4825004"

Revision as of 15:15, 12 October 2023

Cry1518-35

"Cry1518-35 is a crystal protein gene isolated from the strain of Bacillus thuringiensis YBT-1518, performing insecticide bioactivity to nematodes. In our project, we will modify both E.coli and S.cerevisiae to express this part as an effective killing agent, in order to eliminate the parasites inside Giant African Snails, A.cantonensis.

This part has specific toxicity to more than 500 species of insects such as Lepidoptera, Diptera, Coleoptera, Hymenoptera, and Homoptera, as well as protozoa, linear animals, and flat animals. However, it is environmentally friendly and has negligible impacts on other aquatic animals, birds and mammals.

While considering the future parasitic control, Cry1518-35 can provide future iGEM teams that dedicate in achieving ecological balance with more choices of nematode pesticides. "

Usage and Biology

After successful attraction of snails, the elimination of parasitic nematode A. cantonensis in the snails are completed by Cry1518-35 proteins. Cry1518-35 is a crystal protein originally produced in Bacillus thuringiensis. The Cry protein was first expressed in E.coli in large amounts for testing on nematodes which is under active progression. In the construction, we fused codon-optimised Cry coding sequence with 6x His tag and PET28a vector. SDS-page was performed and the result shows that Cry protein was successfully expressed with induction. The result was confirmed in Western Blot analysis.

Cry1518-35 is a crystal protein that is able to eliminate certain nematodes. Its toxicity functions by binding with the cadherin receptor in the nematode's midgut and activating the magnesium ion dependent signal transduction pathway that possibly results in the death of living cells of the nematodes.

Sequence and Features