Difference between revisions of "Part:BBa K283049:Design"

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<partinfo>BBa_K283049 short</partinfo>
 
<partinfo>BBa_K283049 short</partinfo>
  
The BBa_K283016 has been made from this composite. The backbone of this biobrick is based on BBa_J61003. First, we changed the pTet-rbs into pLacI-rbs. Then the cheZ encoding gene was inserted between XbaI and SpeI.  
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The BBa_K283002 has been made from this composite. The backbone of this biobrick is based on BBa_J61003. First, we changed the pTet-rbs into pLacI-rbs. Then the tetR gene with LVA tag sequence was inserted between XbaI and SpeI.  
 
<partinfo>BBa_K283049 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K283049 SequenceAndFeatures</partinfo>
  

Revision as of 23:33, 20 October 2009

pLacI-rbs-tetR(LVA)

The BBa_K283002 has been made from this composite. The backbone of this biobrick is based on BBa_J61003. First, we changed the pTet-rbs into pLacI-rbs. Then the tetR gene with LVA tag sequence was inserted between XbaI and SpeI.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

References