Difference between revisions of "Part:BBa K4615000"

 
Line 1: Line 1:
  
 
__NOTOC__
 
__NOTOC__
−
<partinfo>BBa_K4615000 short</partinfo>
+
<partinfo>sgRNA_F</partinfo>
  
 
This part will amplify the backbone of pTargetF along with targeted tpiA sgRNA sequence to generalize linear PCR fragments, products will be transformed into E.coli later and self-circulaized in T-B18.  
 
This part will amplify the backbone of pTargetF along with targeted tpiA sgRNA sequence to generalize linear PCR fragments, products will be transformed into E.coli later and self-circulaized in T-B18.  

Revision as of 15:09, 12 October 2023


No part name specified with partinfo tag.

This part will amplify the backbone of pTargetF along with targeted tpiA sgRNA sequence to generalize linear PCR fragments, products will be transformed into E.coli later and self-circulaized in T-B18.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 27
    Illegal XbaI site found at 33
    Illegal PstI site found at 45
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 27
    Illegal PstI site found at 45
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 27
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 27
    Illegal XbaI site found at 33
    Illegal PstI site found at 45
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 27
    Illegal XbaI site found at 33
    Illegal PstI site found at 45
  • 1000
    COMPATIBLE WITH RFC[1000]