Difference between revisions of "Part:BBa K4765135"
Siliang Zhan (Talk | contribs) (→Agarose gel electrophoresis) |
Siliang Zhan (Talk | contribs) (→Characterization) |
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| + | ====Successful Protein Expression==== | ||
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| + | | <html><img style="width:200px" src="https://static.igem.wiki/teams/4765/wiki/zsl/protein-gel/dstx.png" alt="contributed by Fudan iGEM 2023"></html> | ||
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| + | | '''Figure 2. SDS-PAGE electrophoresis of BBa K4765135''' | ||
| + | We constructed BBa K4765135 into the pET28a plasmid and transformed it into ''E. coli'' BL21 DE3. This lane represents the leaky expression of BBa K4765135. As indicated by the red arrow, we successfully expressed Rv Dsup, ''H. ex'' mtSSB, SAHS 33020 and XRCC1 in this part. | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 14:54, 12 October 2023
ribozyme connected: Dsup + H. ex mtSSB + SAHS 33020 + XRCC1 + FEN1
Usage and Biology
This part is the combination of all the anti-desiccation and anti-UV proteins, we've transformed it to E .coli BL21 DE3 and test its performance.
Characterization
Agarose gel electrophoresis
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| Figure 1. Agarose gel electrophoresis of PCR products, amplified from bacterial colonies/cultures.
From right lane(3) to left lane(1) indicate the successful construction of Dsup, Dsup + H. ex mtSSB, and Dsup + H. ex mtSSB + SAHS 33020. |
Successful Protein Expression
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| Figure 2. SDS-PAGE electrophoresis of BBa K4765135
We constructed BBa K4765135 into the pET28a plasmid and transformed it into E. coli BL21 DE3. This lane represents the leaky expression of BBa K4765135. As indicated by the red arrow, we successfully expressed Rv Dsup, H. ex mtSSB, SAHS 33020 and XRCC1 in this part. Sequence and Features
Assembly Compatibility:
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