Difference between revisions of "Part:BBa K4815018:Experience"
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===Applications of BBa_K4815018=== | ===Applications of BBa_K4815018=== | ||
| + | We used a synthetic promoter to drive the expression of the YeGFP gene on the same plasmid, while the TEF1 promoter was used in the reverse orientation to drive the expression of the mCherry gene. Additionally, we incorporated a lactose-inducible switch to enhance safety. We utilized flow cytometry to monitor the two fluorescence signals excited by different light channels and analyzed the corresponding data. We plotted the natural logarithm of the ratio of GFP to mCherry (ln(GFP/mCherry)) as a frequency distribution graph to showcase the relative expression strength of different promoters in yeast. The figure bellow shows success in utilizing the plasmids in detecting fluorescence intensity. | ||
| + | <html> | ||
| + | <figure><center> | ||
| + | <img alt="" | ||
| + | src="https://static.igem.wiki/teams/4815/wiki/result/7.png" | ||
| + | width="400" | ||
| + | title=""> | ||
| + | </figure> | ||
| + | </html> | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 14:41, 12 October 2023
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how you used this part and how it worked out.
Applications of BBa_K4815018
We used a synthetic promoter to drive the expression of the YeGFP gene on the same plasmid, while the TEF1 promoter was used in the reverse orientation to drive the expression of the mCherry gene. Additionally, we incorporated a lactose-inducible switch to enhance safety. We utilized flow cytometry to monitor the two fluorescence signals excited by different light channels and analyzed the corresponding data. We plotted the natural logarithm of the ratio of GFP to mCherry (ln(GFP/mCherry)) as a frequency distribution graph to showcase the relative expression strength of different promoters in yeast. The figure bellow shows success in utilizing the plasmids in detecting fluorescence intensity.
User Reviews
UNIQbb8c92d93dfcef57-partinfo-00000001-QINU UNIQbb8c92d93dfcef57-partinfo-00000002-QINU