Difference between revisions of "Part:BBa K4451009"
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A transcriptional inhibitor from phiEco32 phage, which is able to cause growth rate suppression in E. coli. | A transcriptional inhibitor from phiEco32 phage, which is able to cause growth rate suppression in E. coli. | ||
The Gp79 gene encodes a ~10kDa protein that has been shown to bind to the core of the RNA polymerase σ70 holoenzyme, which prevents transcription bubble formation in many the housekeeping genes in E. coli and thus enacts global transcriptional downregulation. Whilst the exact molecular mechanism has yet to be elucidated, it is likely that Gp79 is utilised to switch the specificity of host RNA polymerase to that of phiEco32 late genes in the infection cycle (Savalia et al., 2008). | The Gp79 gene encodes a ~10kDa protein that has been shown to bind to the core of the RNA polymerase σ70 holoenzyme, which prevents transcription bubble formation in many the housekeeping genes in E. coli and thus enacts global transcriptional downregulation. Whilst the exact molecular mechanism has yet to be elucidated, it is likely that Gp79 is utilised to switch the specificity of host RNA polymerase to that of phiEco32 late genes in the infection cycle (Savalia et al., 2008). | ||
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| + | https://static.igem.wiki/teams/4939/wiki/assets/assets/registry-assets/bba-k4451009-meanlog2od.png | ||
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| + | Figure 1: Mean log2(OD against time for gp79 | ||
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Revision as of 14:20, 12 October 2023
phiEco32 gp79
BBa_K4451009 encodes the gp79 protein from phiEco32 phage. This protein has been reported to interact with RNA polymerase and cause inhibition of sigma-70-dependent transcription (Savalia et al., 2008).
Description
A transcriptional inhibitor from phiEco32 phage, which is able to cause growth rate suppression in E. coli. The Gp79 gene encodes a ~10kDa protein that has been shown to bind to the core of the RNA polymerase σ70 holoenzyme, which prevents transcription bubble formation in many the housekeeping genes in E. coli and thus enacts global transcriptional downregulation. Whilst the exact molecular mechanism has yet to be elucidated, it is likely that Gp79 is utilised to switch the specificity of host RNA polymerase to that of phiEco32 late genes in the infection cycle (Savalia et al., 2008).
Figure 1: Mean log2(OD against time for gp79
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]