Difference between revisions of "Part:BBa K4821003"
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<partinfo>BBa_K4821003 short</partinfo> | <partinfo>BBa_K4821003 short</partinfo> | ||
| − | This sequence codes for a modified RdhANp reductive dehalogenase that has been docked to PCB-11 and codon-optimized for expression in E. coli K-12. It was designed as an improvement on part BBa_K4821002, the codon-optimized wild-type RdhANp dehalogenase. | + | This sequence codes for a modified RdhANp reductive dehalogenase that has been docked to PCB-11 and codon-optimized for expression in E. coli K-12. This part contains the following mutations: K295L K310A R312T P314A M315L S423T S425V M426L N455L H457D R555M V556I A563G. It was designed as an improvement on part BBa_K4821002, the codon-optimized wild-type RdhANp dehalogenase to possess high catalytic activity against PCB11. |
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | This is a modified RdhANp with the potential to dechlorinate PCB11 for more efficient breakdown of the compound to acetyl CoA by the biphenyl degradation pathway (bph gene cluster). | ||
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Revision as of 14:20, 12 October 2023
RdhANp_M11_K12
This sequence codes for a modified RdhANp reductive dehalogenase that has been docked to PCB-11 and codon-optimized for expression in E. coli K-12. This part contains the following mutations: K295L K310A R312T P314A M315L S423T S425V M426L N455L H457D R555M V556I A563G. It was designed as an improvement on part BBa_K4821002, the codon-optimized wild-type RdhANp dehalogenase to possess high catalytic activity against PCB11.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1628
Illegal BamHI site found at 312
Illegal BamHI site found at 419 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]