Difference between revisions of "Part:BBa K4683027:Experience"
 
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Gel Experimentation
 
Gel Experimentation
 
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We performed RCA on these six miRNAs (miR-20b, miR-328,miR-146, miR-150, miR-122, miR-30c) and ran the subsequent rolling circle product (RCP) on a 1% agarose gel (see Fig.). The gel exhibited a fluorescent band of DNA very close to the well, indicating that a long strand of DNA greater than 1 kB - our RCP - was produced. Therefore, we can validate that our reaction was successful.  
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We performed RCA on three miRNAs (miR-20b, miR-328,miR-146) and ran the subsequent rolling circle product (RCP) on a 1% agarose gel (see Fig.). The gel exhibited a fluorescent band of DNA very close to the well, indicating that a long strand of DNA greater than 1 kB - our RCP - was produced. Therefore, we can validate that our reaction was successful.
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<html><img src="https://static.igem.wiki/teams/4683/wiki/rca-optimization/screenshot-2023-10-11-at-4-49-47-pm.png"
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<html><img src="https://static.igem.wiki/teams/4683/wiki/human-practices/igem-estrogen-label.png"
 
alt="Figure 1. Image of gel ran with miRNA-1 RCP product; A: eRCA with 40.8 pM miR-1; B: negative control (no enzymes)
 
alt="Figure 1. Image of gel ran with miRNA-1 RCP product; A: eRCA with 40.8 pM miR-1; B: negative control (no enzymes)
 
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Figure. RCP from RCA reaction with miR-20b, miR-328,miR-146, miR-150, miR-122, and miR-30c; visible bands can be seen near the top of the wells.
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Figure. RCP from RCA reaction with miR-20b, miR-328,miR-146a; visible bands can be seen near the top of the wells.
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===User Reviews===
 
===User Reviews===
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Latest revision as of 14:03, 12 October 2023


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K4683027

Lambert_GA 2023
Gel Experimentation
We performed RCA on three miRNAs (miR-20b, miR-328,miR-146) and ran the subsequent rolling circle product (RCP) on a 1% agarose gel (see Fig.). The gel exhibited a fluorescent band of DNA very close to the well, indicating that a long strand of DNA greater than 1 kB - our RCP - was produced. Therefore, we can validate that our reaction was successful.
Figure 1. Image of gel ran with miRNA-1 RCP product; A: eRCA with 40.8 pM miR-1; B: negative control (no enzymes)
Figure. RCP from RCA reaction with miR-20b, miR-328,miR-146a; visible bands can be seen near the top of the wells.


User Reviews

UNIQb0ba796e73d0ee03-partinfo-00000001-QINU UNIQb0ba796e73d0ee03-partinfo-00000002-QINU