Difference between revisions of "Part:BBa K4683023:Experience"
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how you used this part and how it worked out. | how you used this part and how it worked out. | ||
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<b> Lambert_GA 2023</b> | <b> Lambert_GA 2023</b> | ||
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Latest revision as of 14:02, 12 October 2023
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how you used this part and how it worked out.
Applications of BBa_K4683023
Lambert_GA 2023
Gel Experimentation
We performed RCA on three miRNAs (miR-20b, miR-328,miR-146) and ran the subsequent rolling circle product (RCP) on a 1% agarose gel (see Fig.). The gel exhibited a fluorescent band of DNA very close to the well, indicating that a long strand of DNA greater than 1 kB - our RCP - was produced. Therefore, we can validate that our reaction was successful.
Figure. RCP from RCA reaction with miR-20b, miR-328,miR-146a; visible bands can be seen near the top of the wells.
User Reviews
UNIQb44e138ab5d33c7c-partinfo-00000001-QINU UNIQb44e138ab5d33c7c-partinfo-00000002-QINU