Difference between revisions of "Part:BBa K4683024:Experience"
 
Line 4: Line 4:
 
how you used this part and how it worked out.
 
how you used this part and how it worked out.
  
−
===Applications of BBa_K4683024===
+
 
 +
__NOTOC__
 +
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 +
how you used this part and how it worked out.
 +
 
 +
===Applications of BBa K4683024===
 +
<b> Lambert_GA 2023</b>
 
<br>
 
<br>
 
Gel Experimentation
 
Gel Experimentation
 
<br>
 
<br>
−
We performed RCA on these six miRNAs (miR-20b, miR-328,miR-146, miR-150, miR-122, miR-30c) and ran the subsequent rolling circle product (RCP) on a 1% agarose gel (see Fig.). The gel exhibited a fluorescent band of DNA very close to the well, indicating that a long strand of DNA greater than 1 kB - our RCP - was produced. Therefore, we can validate that our reaction was successful.  
+
We performed RCA on three miRNAs (miR-20b, miR-328,miR-146) and ran the subsequent rolling circle product (RCP) on a 1% agarose gel (see Fig.). The gel exhibited a fluorescent band of DNA very close to the well, indicating that a long strand of DNA greater than 1 kB - our RCP - was produced. Therefore, we can validate that our reaction was successful.
−
<br>
+
<br>  
−
<html><img src="https://static.igem.wiki/teams/4683/wiki/rca-optimization/screenshot-2023-10-11-at-4-49-47-pm.png"
+
<html><img src="https://static.igem.wiki/teams/4683/wiki/human-practices/igem-estrogen-label.png"
 
alt="Figure 1. Image of gel ran with miRNA-1 RCP product; A: eRCA with 40.8 pM miR-1; B: negative control (no enzymes)
 
alt="Figure 1. Image of gel ran with miRNA-1 RCP product; A: eRCA with 40.8 pM miR-1; B: negative control (no enzymes)
 
" width="500"></html>
 
" width="500"></html>
 
<br>
 
<br>
−
Figure. RCP from RCA reaction with miR-20b, miR-328,miR-146, miR-150, miR-122, and miR-30c; visible bands can be seen near the top of the wells.
+
Figure. RCP from RCA reaction with miR-20b, miR-328,miR-146a; visible bands can be seen near the top of the wells.
 +
 
 +
 
  
 
===User Reviews===
 
===User Reviews===
−
<!-- DON'T DELETE --><partinfo>BBa_K4683024 StartReviews</partinfo>
+
<!-- DON'T DELETE --><partinfo>BBa_K4683005 StartReviews</partinfo>
 
<!-- Template for a user review
 
<!-- Template for a user review
 
{|width='80%' style='border:1px solid gray'
 
{|width='80%' style='border:1px solid gray'
 
|-
 
|-
 
|width='10%'|
 
|width='10%'|
−
<partinfo>BBa_K4683024 AddReview number</partinfo>
+
<partinfo>BBa_K4683005 AddReview number</partinfo>
 
<I>Username</I>
 
<I>Username</I>
 
|width='60%' valign='top'|
 
|width='60%' valign='top'|
Line 28: Line 36:
 
|};
 
|};
 
<!-- End of the user review template -->
 
<!-- End of the user review template -->
−
<!-- DON'T DELETE --><partinfo>BBa_K4683024 EndReviews</partinfo>
+
<!-- DON'T DELETE --><partinfo>BBa_K4683005 EndReviews</partinfo>

Latest revision as of 14:00, 12 October 2023


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa K4683024

Lambert_GA 2023
Gel Experimentation
We performed RCA on three miRNAs (miR-20b, miR-328,miR-146) and ran the subsequent rolling circle product (RCP) on a 1% agarose gel (see Fig.). The gel exhibited a fluorescent band of DNA very close to the well, indicating that a long strand of DNA greater than 1 kB - our RCP - was produced. Therefore, we can validate that our reaction was successful.
Figure 1. Image of gel ran with miRNA-1 RCP product; A: eRCA with 40.8 pM miR-1; B: negative control (no enzymes)
Figure. RCP from RCA reaction with miR-20b, miR-328,miR-146a; visible bands can be seen near the top of the wells.


User Reviews

UNIQc4bcff93a771f2fb-partinfo-00000001-QINU UNIQc4bcff93a771f2fb-partinfo-00000002-QINU