Difference between revisions of "Part:BBa K4759205:Design"
 
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===Source===
 
===Source===
  
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company
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The solubilization-promoting tag MBP was from Group: iGEM10_Calgary.
  
 
===References===
 
===References===

Latest revision as of 13:59, 12 October 2023


T7-RBS4-MBP-linker-OleP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 446
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1229
    Illegal AgeI site found at 1327
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 144


Design Notes

Olep is selected to synthesize the above gene by adding histidine tags at the N-terminal


Source

The solubilization-promoting tag MBP was from Group: iGEM10_Calgary.

References