Difference between revisions of "Part:BBa K4584003"
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Create LldR expression pSB1C3 vector with strong promoter (BBa_J23100) , strong RBS (BBa_B0034) and LldR from corynebacterial (BBa_K4584000) | Create LldR expression pSB1C3 vector with strong promoter (BBa_J23100) , strong RBS (BBa_B0034) and LldR from corynebacterial (BBa_K4584000) | ||
| − | We designed and ordered a new composite BioBrick BBa_K4584003 with cloning linkers | + | We designed and ordered a new composite BioBrick BBa_K4584003 with cloning linkers, strong promoter BioBrick BBa_J23100, strong RBS BioBrick BBa_B0034 and LldR from corynebacterial BioBrick BBa_K45840000. |
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The pSB1C3 vector, containing a strong promoter, strong RBS, and the corynebacterial LldR transcription factor, is crafted for expression of LldR in bacteria. This engineered plasmid allows for in detailed studies on its function and potential regulatory roles, particularly pertaining to lactate metabolism. | The pSB1C3 vector, containing a strong promoter, strong RBS, and the corynebacterial LldR transcription factor, is crafted for expression of LldR in bacteria. This engineered plasmid allows for in detailed studies on its function and potential regulatory roles, particularly pertaining to lactate metabolism. | ||
Latest revision as of 13:57, 12 October 2023
pSB1C3 vector with strong promoter, strong RBS, corynebacteria LldR
Create LldR expression pSB1C3 vector with strong promoter (BBa_J23100) , strong RBS (BBa_B0034) and LldR from corynebacterial (BBa_K4584000)
We designed and ordered a new composite BioBrick BBa_K4584003 with cloning linkers, strong promoter BioBrick BBa_J23100, strong RBS BioBrick BBa_B0034 and LldR from corynebacterial BioBrick BBa_K45840000.
The pSB1C3 vector, containing a strong promoter, strong RBS, and the corynebacterial LldR transcription factor, is crafted for expression of LldR in bacteria. This engineered plasmid allows for in detailed studies on its function and potential regulatory roles, particularly pertaining to lactate metabolism.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 15
Illegal NheI site found at 38 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 256
Illegal XhoI site found at 478 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 624
- 1000COMPATIBLE WITH RFC[1000]