Difference between revisions of "Part:BBa K4790071"

Latest revision as of 13:54, 12 October 2023

ASR6(node 112)

Node 112 is the ancestral sequence of PETase predicted by constructing a phylogenetic tree using specific algorithm based on some enzymes that have been reported to be capable of hydrolyzing plastics. That is to say, it is an ancient protein that we resurrected, and it is speculated to have excellent properties such as good temperature stability and mutation stability. In this project, we will use machine learning for prediction, mutate node 112, and find variants with excellent properties.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 281
Illegal PstI site found at 292
Illegal PstI site found at 418
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 268
Illegal PstI site found at 281
Illegal PstI site found at 292
Illegal PstI site found at 418
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 288
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 281
Illegal PstI site found at 292
Illegal PstI site found at 418
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 281
Illegal PstI site found at 292
Illegal PstI site found at 418
Illegal NgoMIV site found at 264
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K4790071 short</partinfo>
-Node 112 is the ancestral sequence of PETase predicted by constructing a phylogenetic tree using specific algorithm based on some enzymes that have been reported to be capable of hydrolyzing plastics. That is to say, it is an ancient protein that we resurrected, and it is speculated to have excellent properties such as good temperature stability and mutation stability. In this project, we will use machine learning for prediction, mutate node112, and find variants with excellent properties.
+Node 112 is the ancestral sequence of PETase predicted by constructing a phylogenetic tree using specific algorithm based on some enzymes that have been reported to be capable of hydrolyzing plastics. That is to say, it is an ancient protein that we resurrected, and it is speculated to have excellent properties such as good temperature stability and mutation stability. In this project, we will use machine learning for prediction, mutate node 112, and find variants with excellent properties.
 <!-- Add more about the biology of this part here