Difference between revisions of "Part:BBa K4790064"

Latest revision as of 13:52, 12 October 2023

ASR1(node 59)

Node 59 is the ancestral sequence of PETase predicted by constructing a phylogenetic tree using specific algorithm based on some enzymes that have been reported to be capable of hydrolyzing plastics. That is to say, it is an ancient protein that we resurrected, and it is speculated to have excellent properties such as good temperature stability and mutation stability. In this project, we will use machine learning for prediction, mutate node 59, and find variants with excellent properties.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 799
Illegal PstI site found at 100
Illegal PstI site found at 232
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 799
Illegal NheI site found at 721
Illegal PstI site found at 100
Illegal PstI site found at 232
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 799
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 799
Illegal PstI site found at 100
Illegal PstI site found at 232
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 799
Illegal PstI site found at 100
Illegal PstI site found at 232
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4790064 short</partinfo>
-Node 59 is the ancestral sequence of PETase predicted by constructing a phylogenetic tree using specific algorithm based on some enzymes that have been reported to be capable of hydrolyzing plastics. That is to say, it is an ancient protein that we resurrected, and it is speculated to have excellent properties such as good temperature stability and mutation stability. In this project, we will use machine learning for prediction, mutate node59, and find variants with excellent properties.
+Node 59 is the ancestral sequence of PETase predicted by constructing a phylogenetic tree using specific algorithm based on some enzymes that have been reported to be capable of hydrolyzing plastics. That is to say, it is an ancient protein that we resurrected, and it is speculated to have excellent properties such as good temperature stability and mutation stability. In this project, we will use machine learning for prediction, mutate node 59, and find variants with excellent properties.
 <!-- Add more about the biology of this part here