Difference between revisions of "Part:BBa K4683016:Experience"
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<b> Lambert_GA 2023</b>
 
<b> Lambert_GA 2023</b>
 
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Gel Experimentation
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We characterized the padlock and the miRNA sequence, but unfortunately, we were unable to characterize these parts due to lack of time.  
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We performed RCA on these six miRNAs (miR-20b, miR-328,miR-146, miR-150, miR-122, miR-30c) and ran the subsequent rolling circle product (RCP) on a 1% agarose gel (see Fig.). The gel exhibited a fluorescent band of DNA very close to the well, indicating that a long strand of DNA greater than 1 kB - our RCP - was produced. Therefore, we can validate that our reaction was successful.
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<html><img src="https://static.igem.wiki/teams/4683/wiki/rca-optimization/screenshot-2023-10-11-at-4-49-47-pm.png"
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alt="Figure 1. Image of gel ran with miRNA-1 RCP product; A: eRCA with 40.8 pM miR-1; B: negative control (no enzymes)
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Figure. RCP from RCA reaction with miR-20b, miR-328,miR-146, miR-150, miR-122, and miR-30c; visible bands can be seen near the top of the wells.
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Revision as of 13:51, 12 October 2023


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Please enter how you used this part and how it worked out.

Applications of BBa_K4683016

Lambert_GA 2023
We characterized the padlock and the miRNA sequence, but unfortunately, we were unable to characterize these parts due to lack of time.


User Reviews

UNIQ8c1aeab55c140279-partinfo-00000000-QINU UNIQ8c1aeab55c140279-partinfo-00000001-QINU