Difference between revisions of "Part:BBa K4601202:Design"
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===Source=== | ===Source=== | ||
+ | |||
+ | DNA synthesis, PCR amplifications and Golden Gate assembly | ||
===References=== | ===References=== |
Latest revision as of 13:46, 12 October 2023
Jaws expression cassette in the Evolution.T7 mutational region
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 880
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1115
Illegal AgeI site found at 1140 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Source
DNA synthesis, PCR amplifications and Golden Gate assembly