Difference between revisions of "Part:BBa K174015"

 
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This Cadmium sensor has binding sites for ArsR and CzrA metal sensors. By placing them together, our device works as an AND gate and senses Cadmium when both proteins bind to Cadmium.
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This cadmium sensor is built with binding sites for two metal sensor proteins, ArsR and CzrA (1,2). They both work as repressors and they are relieved from binding to the DNA when they are bound to various metals. ArsR can bind to cadmium, silver, copper and arsenic whereas CzrA can bind to zinc, cobalt, nickel and cadmium. Cadmium can bind to both proteins and using a combinatorial approach enables us to filter out sensing other metals rather than cadmium.
  
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When Cadmium is not present both proteins bind to their relevant sites on the promoter and represses the expression of downstream genes. When Cadmium present in the environment, it binds to these repressors which then fall of from where they are bound to on the promoter. Hence the promoter becomes free to express the downstream genes as an indication of Cadmium.
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When cadmium is present both proteins are unbound from this AND gate and the promoter region controlled by the repressors becomes free to drive the expression of downstream genes.
  
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This combinatorial approach enables us to filter out sensing other metals. ArsR and CzrA can bind to different metals, however when both are used as an ANd gate, they will only recognize Cadmium.
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For more information about this part, go to Newcastle iGEM 2009 [http://2009.igem.org/Team:Newcastle/Project/Overview
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Overview] and [http://2009.igem.org/Team:Newcastle/Metalsensing Metal Sensing] pages.
  
 
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===References===
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#Moore, C. M. and J. D. Helmann (2005). "Metal ion homeostasis in Bacillus subtilis." Current Opinion in Microbiology 8(2): 188-195.
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#Harvie, D.R., et al., Predicting metals sensed by ArsR-SmtB repressors: Allosteric interference by a non-effector metal. Molecular Microbiology, 2006. 59(4): p. 1341-1356.

Revision as of 22:21, 20 October 2009

Cadmium Sensor

This cadmium sensor is built with binding sites for two metal sensor proteins, ArsR and CzrA (1,2). They both work as repressors and they are relieved from binding to the DNA when they are bound to various metals. ArsR can bind to cadmium, silver, copper and arsenic whereas CzrA can bind to zinc, cobalt, nickel and cadmium. Cadmium can bind to both proteins and using a combinatorial approach enables us to filter out sensing other metals rather than cadmium.

When cadmium is present both proteins are unbound from this AND gate and the promoter region controlled by the repressors becomes free to drive the expression of downstream genes.

For more information about this part, go to Newcastle iGEM 2009 [http://2009.igem.org/Team:Newcastle/Project/Overview 

Overview] and [http://2009.igem.org/Team:Newcastle/Metalsensing Metal Sensing] pages.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 200
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

  1. Moore, C. M. and J. D. Helmann (2005). "Metal ion homeostasis in Bacillus subtilis." Current Opinion in Microbiology 8(2): 188-195.
  2. Harvie, D.R., et al., Predicting metals sensed by ArsR-SmtB repressors: Allosteric interference by a non-effector metal. Molecular Microbiology, 2006. 59(4): p. 1341-1356.