Difference between revisions of "Part:BBa K4591011"
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<partinfo>BBa_K4591011 short</partinfo>
 
<partinfo>BBa_K4591011 short</partinfo>
 
===Usage and Biology===
 
===Usage and Biology===
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Our system was to be able to work perfectly in the environment, but we have to consider that the trigger conditions of the suicide switch are not suitable for the stage of cultivation and modification of engineered bacteria, since the concentrations of engineered bacteria need to be high at that stage. Therefore, it is imperative to investigate novel methods for regulating the suicide switch.
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<p>Our system was to be able to work perfectly in the environment, but we have to consider that the trigger conditions of the suicide switch are not suitable for the stage of cultivation and modification of engineered bacteria, since the concentrations of engineered bacteria need to be high at that stage. Therefore, it is imperative to investigate novel methods for regulating the suicide switch.</p>
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The method of homologous recombination also brought us into contacting with the topic of recombinases.
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<p>Cre-Loxp recombination is a site-specific recombinase technology, which was used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. The effectiveness of this technology hinges on the presence of flanking sequences, known as Recombinase Target Sites (RTSs), that mark recombination sites.If the RTSs are arranged in head-to-head orientation, the sequence within the RTSs is flipped (called inversion).</p><p>
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Cre-Loxp recombination is a site-specific recombinase technology, which was used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. The effectiveness of this technology hinges on the presence of flanking sequences, known as Recombinase Target Sites (RTSs), that mark recombination sites.If the RTSs are arranged in head-to-head orientation, the sequence within the RTSs is flipped (called inversion).
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With this Flip Module, we can precisely control the on and off of the Suicide Switch by ingenious genetic circuit design and inducing the expression of cre recombinase. </p>
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With this Flip Module, we can precisely control the on and off of the Suicide Switch by ingenious genetic circuit design and inducing the expression of cre recombinase.
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===Charcterization===
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 13:36, 12 October 2023


T500-Para-lox66-PdegQ-lox71-MazF-T500

Usage and Biology

Our system was to be able to work perfectly in the environment, but we have to consider that the trigger conditions of the suicide switch are not suitable for the stage of cultivation and modification of engineered bacteria, since the concentrations of engineered bacteria need to be high at that stage. Therefore, it is imperative to investigate novel methods for regulating the suicide switch.

Cre-Loxp recombination is a site-specific recombinase technology, which was used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. The effectiveness of this technology hinges on the presence of flanking sequences, known as Recombinase Target Sites (RTSs), that mark recombination sites.If the RTSs are arranged in head-to-head orientation, the sequence within the RTSs is flipped (called inversion).

With this Flip Module, we can precisely control the on and off of the Suicide Switch by ingenious genetic circuit design and inducing the expression of cre recombinase.

Charcterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 324
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 155
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 137