Difference between revisions of "Part:BBa K4614002:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | R5 is a short 19-amino acid peptide with multiple Lys catalytic groups that plays an important role in the formation of the siliceous cell wall of diatoms, and we wanted to use it to hydrolyze TEOS to generate silica and deposit silica on the surface of R5.INP is an ice nucleoprotein encoded by Lnak from Pseudomonas syring, which we use to surface R5 with E. coli. | + | R5 is a short 19-amino acid peptide with multiple Lys catalytic groups that plays an important role in the formation of the siliceous cell wall of diatoms, and we wanted to use it to hydrolyze TEOS to generate silica and deposit silica on the surface of R5.INP(encoded by Lnak) is an ice nucleoprotein encoded by Lnak from Pseudomonas syring, which we use to surface R5 with E. coli. |
===Source=== | ===Source=== | ||
Revision as of 13:33, 12 October 2023
Lnak-R5
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 529
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 529
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 529
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 529
Illegal NgoMIV site found at 72
Illegal NgoMIV site found at 405
Illegal AgeI site found at 679 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
R5 is a short 19-amino acid peptide with multiple Lys catalytic groups that plays an important role in the formation of the siliceous cell wall of diatoms, and we wanted to use it to hydrolyze TEOS to generate silica and deposit silica on the surface of R5.INP(encoded by Lnak) is an ice nucleoprotein encoded by Lnak from Pseudomonas syring, which we use to surface R5 with E. coli.
Source
IPN comes from the genome of Pseudomonas syringae.
References
[1]薛双红.(2017).基于细菌表面展示技术的功能性无机材料合成研究(硕士学位论文,武汉理工大学).https://kns.cnki.net/KCMS/detail/detail.aspx?dbname=CMFD201901&filename=1019808772.nh