Difference between revisions of "Part:BBa K4800062"

 
 
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<partinfo>BBa_K4800062 short</partinfo>
 
<partinfo>BBa_K4800062 short</partinfo>
  
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The genes of certain protein scaffolds including SpyTag, EutM and SpyCatcher were subcloned into the plasmid pRSFDuet-sfp-MmCARQ302E-YahK together to yield plasmid pRSFDuet-sfp-MmCARQ302E-SpyTag-EutM-SpyCatcher-YahK.  
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The genes of certain protein scaffolds including SpyTag, EutM and SpyCatcher were subcloned into the plasmid pRSFDuet-sfp-MmCAR(Q302E)-YahK together to yield plasmid pRSFDuet-sfp-MmCAR(Q302E)-SpyTag-EutM-SpyCatcher-YahK.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 13:12, 12 October 2023


pRSFDuet-sfp-MmCAR(Q302E)-SpyTag-EutM-SpyCatcher-YahK

The genes of certain protein scaffolds including SpyTag, EutM and SpyCatcher were subcloned into the plasmid pRSFDuet-sfp-MmCAR(Q302E)-YahK together to yield plasmid pRSFDuet-sfp-MmCAR(Q302E)-SpyTag-EutM-SpyCatcher-YahK.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Unknown
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    INCOMPATIBLE WITH RFC[23]
    Unknown
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4393
    Illegal EcoRI site found at 8178
    Illegal XbaI site found at 1675
    Illegal XbaI site found at 8096
    Illegal SpeI site found at 3076
    Illegal SpeI site found at 3607
    Illegal PstI site found at 4828
    Illegal PstI site found at 4855
    Illegal PstI site found at 6124
    Illegal PstI site found at 6151
    Illegal PstI site found at 6544
    Illegal PstI site found at 7040
    Illegal PstI site found at 7684
    Illegal NgoMIV site found at 6652
    Illegal NgoMIV site found at 6714
    Illegal NgoMIV site found at 6931
    Illegal NgoMIV site found at 7378
    Illegal AgeI site found at 4558
    Illegal AgeI site found at 5569
    Illegal AgeI site found at 5866
    Illegal AgeI site found at 6751
    Illegal AgeI site found at 6760
    Illegal AgeI site found at 7435
    Illegal AgeI site found at 10200
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 10273
    Illegal SapI site found at 920