Difference between revisions of "Part:BBa K174008:Design"

 
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===Design Notes===
 
===Design Notes===
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It is integrated to ''B. subtilis'' using the homology between cotC fusion and the native copy of cotC on the chromosome.  
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It can be integrated to ''B. subtilis'' using the homology between cotC fusion and the native copy of cotC on the chromosome.  
  
  

Revision as of 21:37, 20 October 2009

SmtA metallothionein protein with CotC and Gfp fusion


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1234
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1046


Design Notes

It can be integrated to B. subtilis using the homology between cotC fusion and the native copy of cotC on the chromosome.


Source

smtA sequence is taken from E. coli

References