Difference between revisions of "Part:BBa K225001:Design"

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===Design Notes===
 
===Design Notes===
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ScaS/EnvZ fusion was designed by combining the light receptor domain of ScaS (phytochrome from Synechocystis sp PCC 6803) and EnvZ.
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ScaS/EnvZ fusion was designed by combining the light receptor domain of ScaS (chromatic acclimation sensor from Synechocystis sp PCC 6803) with EnvZ.
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I mean that I changed the kinase domain of ScaS to EnvZ to make it work in E coli.
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The histidine kinase domain in ScaS was changed to EnvZ.
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===Source===
 
===Source===

Latest revision as of 21:34, 20 October 2009

Green light activated actuator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2202
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1564
    Illegal BglII site found at 2456
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1117
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

ScaS/EnvZ fusion was designed by combining the light receptor domain of ScaS (chromatic acclimation sensor from Synechocystis sp PCC 6803) with EnvZ. The histidine kinase domain in ScaS was changed to EnvZ.

Source

ScaS from Synechocysitis sp. PCC 6803

References

Hirose et al. (2008) Cyanobacteriochrome CcaS is the green light receptor that induces the expression of phycobilisome linker protein. Proc Natl Acad Sci U S A.105(28), 9528-33