Difference between revisions of "Part:BBa K4765127"
Siliang Zhan (Talk | contribs) (→Characterization) |
|||
| Line 8: | Line 8: | ||
Similar to [https://parts.igem.org/Part:BBa_K4765126 BBa_K4765126(ribozyme connected: ''H. ex'' mtSSB + AnAFP + SAHS 33020)], we changed the order of ''H. ex'' mtSSB, SAHS 33020 and AnAFP. | Similar to [https://parts.igem.org/Part:BBa_K4765126 BBa_K4765126(ribozyme connected: ''H. ex'' mtSSB + AnAFP + SAHS 33020)], we changed the order of ''H. ex'' mtSSB, SAHS 33020 and AnAFP. | ||
===Characterization=== | ===Characterization=== | ||
| − | Get details in [https://parts.igem.org/Part:BBa_K4765126 BBa_K4765126 | + | Get details in [https://parts.igem.org/Part:BBa_K4765126 BBa_K4765126]. |
====Agarose gel electrophoresis==== | ====Agarose gel electrophoresis==== | ||
{| | {| | ||
Revision as of 12:59, 12 October 2023
ribozyme connected: H. ex mtSSB + AnAFP + SAHS 33020
Introduction
Similar to BBa_K4765126(ribozyme connected: H. ex mtSSB + AnAFP + SAHS 33020), we changed the order of H. ex mtSSB, SAHS 33020 and AnAFP.
Characterization
Get details in BBa_K4765126.
Agarose gel electrophoresis
|
| Figure 1. Agarose gel electrophoresis of PCR products, amplified from bacterial colonies/cultures.
From left lane(1) to right lane(3) indicate the successful construction of H. ex mtSSB, H. ex mtSSB + AnAFP, and H. ex mtSSB + AnAFP + SAHS 33020. |
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 247
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1573
Illegal BsaI.rc site found at 515
Illegal BsaI.rc site found at 596
Illegal SapI site found at 409
Illegal SapI.rc site found at 1214