Difference between revisions of "Part:BBa K225001"

Revision as of 21:29, 20 October 2009

Green light activated actuator

Green light activated actuator composed of the phycocyanobilin synthesis genes (ho1 and pcyA) and the green light receptor (GlrN).

Usage and Biology

GlrN is the fusion protein of light responsive domains of CcaS and EnvZ designed by changing the histidine kinase region of CcaS to EnvZ domain of cph8 (BBa_I15010).

Requires phycocyanobilin biosynthetic genes (BBa_I15008 and BBa_I15009) for chromophore formation.
Must be used in E. coli deficient in natural EnvZ.

Expecting function (without experimental proof)

Exposure to green light (535 nm) phosphorylate EnvZ histidin kinase domain. The activated EnvZ phosphorylates endogenous OmpR, a transcription factor which activates transcription from the OmpR positive promoter (BBa_R0083).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 2202
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1564
Illegal BglII site found at 2456
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1117
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K225001 short</partinfo>
-RBS-ho1-RBS-pcyA-RBS-GlrN-Trem
+Green light activated actuator composed of the phycocyanobilin synthesis genes (ho1 and pcyA) and the green light receptor (GlrN).
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+GlrN is the fusion protein of light responsive domains of CcaS and EnvZ designed by changing the histidine kinase region of CcaS to EnvZ domain of cph8 (BBa_I15010).
+* Requires phycocyanobilin biosynthetic genes (BBa_I15008 and BBa_I15009) for chromophore formation.
+* Must be used in E. coli deficient in natural EnvZ.
+===Expecting function (without experimental proof)===
+Exposure to green light (535 nm) phosphorylate EnvZ histidin kinase domain. The activated EnvZ phosphorylates endogenous OmpR, a transcription factor which activates transcription from the OmpR positive promoter (BBa_R0083).
 <!-- -->