Difference between revisions of "Part:BBa K4765134"

Revision as of 12:57, 12 October 2023

ribozyme connected: Dsup + FEN1 + H. ex mtSSB + SAHS 33020 + XRCC1

contributed by Fudan iGEM 2023

Usage and Biology

This part is the combination of all the anti-desiccation and anti-UV proteins, we've transformed it to E .coli BL21 DE3 and test its performance.

Characterization

Agarose gel electrophoresis

Figure 1. Agarose gel electrophoresis of PCR products, amplified from bacterial colonies/cultures.

From right lane(3) to left lane(1) indicate the successful construction of Fen 1 + H. ex mtSSB, Fen 1 + H. ex mtSSB + SAHS 33020, and Fen 1 + H. ex mtSSB + SAHS 33020 + XRCC1.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 4573
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1886
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 2464
Illegal NgoMIV site found at 2782
Illegal AgeI site found at 230
Illegal AgeI site found at 301
Illegal AgeI site found at 1570
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 3415
Illegal BsaI.rc site found at 227
Illegal BsaI.rc site found at 3050
Illegal BsaI.rc site found at 3131
Illegal BsaI.rc site found at 5623
Illegal SapI site found at 2944
Illegal SapI.rc site found at 956

@@ Line 13: / Line 13: @@
 |-
 | '''Figure 1. Agarose gel electrophoresis of PCR products, amplified from bacterial colonies/cultures.
-From right lane(3) to left lane(3) indicate the successful construction of Fen 1 + H. ex mtSSB, Fen 1 + H. ex mtSSB + SAHS 33020, and Fen 1 + H. ex mtSSB + SAHS 33020 + XRCC1. '''
+From right lane(3) to left lane(1) indicate the successful construction of Fen 1 + ''H. ex'' mtSSB, Fen 1 + ''H. ex'' mtSSB + SAHS 33020, and Fen 1 + ''H. ex'' mtSSB + SAHS 33020 + XRCC1. '''
 |}