Difference between revisions of "Part:BBa K4683006:Experience"
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===Applications of BBa_K4683006===
 
===Applications of BBa_K4683006===
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<b> Lambert_GA 2022</b>
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<b> Taken from <partinfo>BBa_K4683005</partinfo> </b>
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Gel Experimentation
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We performed RCA on these six miRNAs (miR-20b, miR-328,miR-146, miR-150, miR-122, miR-30c) and ran the subsequent rolling circle product (RCP) on a 1% agarose gel (see Fig.). The gel exhibited a fluorescent band of DNA very close to the well, indicating that a long strand of DNA greater than 1 kB - our RCP - was produced. Therefore, we can validate that our reaction was successful.
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<html><img src="https://static.igem.wiki/teams/4683/wiki/rca-optimization/screenshot-2023-10-11-at-4-49-47-pm.png"
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alt="Figure 1. Image of gel ran with miRNA-1 RCP product; A: eRCA with 40.8 pM miR-1; B: negative control (no enzymes)
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Figure. RCP from RCA reaction with miR-20b, miR-328,miR-146, miR-150, miR-122, and miR-30c; visible bands can be seen near the top of the wells.
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===User Reviews===
 
===User Reviews===

Revision as of 12:21, 12 October 2023


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K4683006

Taken from BBa_K4683005

User Reviews

UNIQ11aeb4d537cb2169-partinfo-00000001-QINU UNIQ11aeb4d537cb2169-partinfo-00000002-QINU