Difference between revisions of "Part:BBa K4825048:Design"
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===Source=== | ===Source=== | ||
| − | Saccharomyces cerevisiae | + | <i>Saccharomyces cerevisiae</i> |
===References=== | ===References=== | ||
Latest revision as of 12:20, 12 October 2023
COX6-LEU2
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 151
Illegal EcoRI site found at 1090 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 151
Illegal EcoRI site found at 1090 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 151
Illegal EcoRI site found at 1090 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 151
Illegal EcoRI site found at 1090 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 151
Illegal EcoRI site found at 1090
Illegal AgeI site found at 703 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 437
Illegal SapI.rc site found at 421
Design Notes
The composite part appends the first 41 amino acids of COX6 to the N-terminus of LEU2 in order to relocate and overexpress LEU2 in mitochondria.
Source
Saccharomyces cerevisiae
References
1. ACS Synthetic Biology 2020 9 (3), 546-555 2. "ACS Synth. Biol. 2020, 9, 3, 546–555 Publication Date:February 12, 2020