Difference between revisions of "Part:BBa K4790032"
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<partinfo>BBa_K4790032 short</partinfo> | <partinfo>BBa_K4790032 short</partinfo> | ||
− | Mutate K249 of ASR1 ( | + | Mutate K249 of ASR1 (node 59) to E249 by site-directed mutagenesis. |
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Latest revision as of 12:13, 12 October 2023
ASR1(node 59)-K249E
Mutate K249 of ASR1 (node 59) to E249 by site-directed mutagenesis.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 799
Illegal PstI site found at 100
Illegal PstI site found at 232 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 799
Illegal NheI site found at 721
Illegal PstI site found at 100
Illegal PstI site found at 232 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 799
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 799
Illegal PstI site found at 100
Illegal PstI site found at 232 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 799
Illegal PstI site found at 100
Illegal PstI site found at 232 - 1000COMPATIBLE WITH RFC[1000]