Difference between revisions of "Part:BBa K4451011"

Revision as of 12:00, 12 October 2023

77 gp104

BBa_K4451008 encodes the gp104 protein from the 77 phage. This protein has been reported to interact with DnaI (helicase loader) and shuts down host replication (Liu et al., 2004).

Description

An inhibitor of DNA replication from the 77 phage, which causes growth rate suppression in Staphylococcus aureus. The gp104 gene encodes a small inhibitory protein that targets and inhibits the DnaI protein in S. aureus, itself responsible for helicase loading onto replicating DNA and thus a crucial part of the replisome. Its inhibition shows potent growth rate suppression due to the stalling of DNA replication initiation. To date, no evidence has been shown to suggest its function in E. coli, and it is worth noting that E. coli itself does not have a closely related DnaI homolog (Liu et al., 2004).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 BBa_K4451008 encodes the gp104 protein from the 77 phage. This protein has been reported to interact with DnaI (helicase loader) and shuts down host replication (Liu et al., 2004).
+===Description===
+An inhibitor of DNA replication from the 77 phage, which causes growth rate suppression in Staphylococcus aureus.
+The gp104 gene encodes a small inhibitory protein that targets and inhibits the DnaI protein in S. aureus, itself responsible for helicase loading onto replicating DNA and thus a crucial part of the replisome. Its inhibition shows potent growth rate suppression due to the stalling of DNA replication initiation. To date, no evidence has been shown to suggest its function in E. coli, and it is worth noting that E. coli itself does not have a closely related DnaI homolog (Liu et al., 2004).
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